Sign in →

Test Code ACARP Acanthamoeba species Molecular Detection, PCR, Ocular

Useful For

Aid in the diagnosis of amebic keratitis in conjunction with clinical findings

Method Name

Real-Time Polymerase Chain Reaction (PCR)/TaqMan DNA Probe Hybridization
(PCR is utilized pursuant to a license agreement with Roche Molecular Systems, Inc.) 

Reporting Name

Acanthamoeba species Detection, PCR

Specimen Type

Varies


Specimen Required


Specimen source is required.

 

Specimens collected using wooden shafted swabs will be canceled.

 

Fluorescein dye may interfere with testing and should not be used prior to collection. Specimens containing fluorescein dye will be canceled.

 

The preferred specimen for Acanthamoeba PCR from an ocular source is corneal scraping or biopsy.

 

Submit only 1 of the following specimens: 

 

Specimen Type: Tissue: Fresh

Sources: Ocular

Container/Tube: Sterile container

Specimen Volume: 5-10 mm

Collection Instructions: Submit tissue in a sterile container with 1 mL of sterile saline, minimal essential media (MEM), or viral transport media.  

    

Specimen Type: Tissue: Formalin-fixed paraffin-embedded (FFPE)

Sources: Ocular

Container/Tube: Sterile container       

Specimen Volume: 5-10 mm

Collection Instructions: Cut tissue into five 10-micron sections and place in a sterile container.  

 

Specimen Type: Scrapings/Swabs

Sources: Eye, ocular, cornea

Container/Tube: Sterile container

Specimen Volume: 1 mL

Collection Instructions:

1. Collect corneal scrapings using a scalpel or other sharp device to remove the outer layer of cells from the eye.

2. Swish the collection device in 1 mL of sterile saline, minimal essential media (MEM) or viral transport media.

3. Remove the collection device from the collection container before submitting to the lab.

4. Specimens containing scalpel blades will be canceled.

Additional Information: Swabs are not the preferred specimen for this test and may yield false-negative results.

 

Specimen Type: Contact lenses

Container/Tube: Sterile container

Specimen Volume: Entire collection

Collection Instructions:

1. Place entire contact lens in a sterile container with 1 mL sterile saline, contact lens solution, viral transport media, or minimal essential media (MEM).  

2. Right and Left lenses must be submitted individually using multiple sterile containers or in the original contact lens case. Multiple orders must be created.  

3. Indicate Right or Left in the specimen source.

 

Specimen Type: Contact lens solution

Container/Tube: Sterile container

Specimen Volume: 1 mL solution

 

Specimen Type: Contact lens cases without lenses

Container/Tube: Sterile container

Specimen Volume: 1 mL solution or entire case

Additional Information:

1. Depending on the type of case submitted, it may be necessary to test right and left chambers individually. Multiple orders must be created.

2. Indicate Right or Left in the specimen source.


Specimen Minimum Volume

Tissue: 5 mm biopsy; Scrapings: 0.5 mL; Contact Lens Solution: 1 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated 7 days

Reference Values

Negative

Day(s) and Time(s) Performed

Monday through Saturday

Performing Laboratory

Mayo Medical Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ACARP Acanthamoeba species Detection, PCR 41429-2

 

Result ID Test Result Name Result LOINC Value
SRCAS Specimen Source 31208-2
38058 Acanthamoeba species PCR 41429-2