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Test Code BARRP Bartonella, Molecular Detection, PCR

Reporting Name

Bartonella PCR

Useful For

Diagnosing Bartonella infection

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Advisory Information


BART / Bartonella Antibody Panel, IgG and IgM, Serum and Warthin-Starry tissue stain (PATHC / Pathology Consultation) should be considered if PCR is negative and there is a strong suspicion of disease caused by these organisms.



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by Bartonella species DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Specimen Type: Fresh tissue or biopsy

Sources: Heart valve, liver, lymph node, or spleen

Container/Tube: Sterile container

Specimen Volume: Entire collection or 5 mm(3)

Collection Instructions: Collect fresh tissue specimen.

Specimen Stability Information: Refrigerated (preferred) <7 days/ Frozen <7 days  

 

Supplies: Tissue Block Container (T553)

Specimen Type: Paraffin-embedded tissue block

Sources: Heart valve, liver, lymph node, or spleen

Container/Tube: Sterile container for cut sections or Tissue Block Container (T553)

Specimen Volume: Submit formalin-fixed, paraffin-embedded tissue block to be cut and returned. Alternatively, perform cuts and place two to five 10-micron sections in a sterile container for submission

Specimen Stability Information: Ambient (preferred)/Refrigerated

 

Specimen Type: Fluid

Sources: Cerebrospinal or ocular (eg, vitreous humor fluid)

Container/Tube: Sterile vial

Specimen Volume: 0.5 mL

Specimen Stability Information: Refrigerated (preferred) <7 days/Frozen <7 days

 

Specimen Type: Synovial fluid

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Pink top (EDTA), royal blue top (EDTA), sterile vial containing EDTA-derived aliquot, red clot tube (no anticoagulant), or sterile container

Specimen Volume: 0.5 mL

Collection Instructions: Send specimen in original tube (preferred).

Specimen Stability Information: Refrigerated <7 days (preferred)/Frozen <7 days


Specimen Minimum Volume

Fresh tissue or biopsy: 5 mm(3)
Paraffin-embedded tissue block: two 10-micron sections
Fluid: 0.5 mL

Specimen Stability Information

Specimen Type Temperature Time
Varies Varies

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday, Wednesday, Friday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87801

LOINC Code Information

Test ID Test Order Name Order LOINC Value
BARRP Bartonella PCR 48864-3

 

Result ID Test Result Name Result LOINC Value
SRC51 Specimen source 31208-2
84440 Bartonella PCR 48864-3

Method Name

Rapid Polymerase Chain Reaction (PCR)

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request Form (T244) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/microbiology_test_request_form.pdf)

Testing Algorithm

See Infective Endocarditis: Diagnostic Testing for Identification of Microbiological Etiology in Special Instructions.