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Test Code FRAG Osmotic Fragility, Erythrocytes

Reporting Name

Osmotic Fragility, RBC

Useful For

Evaluation of suspected hereditary spherocytosis associated hemolytic anemia

 

Confirming or detecting mild spherocytosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRAGO Osmotic Fragility No Yes
SCTRL Shipping Control Vial No Yes

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Control
Whole Blood EDTA


Advisory Information


A whole blood EDTA specimen and a control specimen are required.



Additional Testing Requirements


 



Shipping Instructions


Specimens must arrive within 72 hours of draw.



Specimen Required


Patient:

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

2. Send specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

4. Be sure specimen and control are stored and transported together at refrigerate temperature, carefully following proper handling and shipping instructions.

Additional Information: Patient's age and sex are required.

 

Normal Shipping Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Label clearly on outermost label normal control.

3. Immediately refrigerate specimen after draw. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Control Refrigerated 72 hours
Whole Blood EDTA Refrigerated 72 hours

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

 

Reference values have not been established for patients who are <12 months of age.

Day(s) and Time(s) Performed

Monday through Saturday

Test Classification

See Individual Test IDs

CPT Code Information

85557

LOINC Code Information

Test ID Test Order Name Order LOINC Value
FRAG Osmotic Fragility, RBC In Process

 

Result ID Test Result Name Result LOINC Value
9064 Osmotic Fragility, RBC 34964-7
SCTRL Shipping Control Vial 40431-9
3306 Osmotic Fragility, 0.50 g/dL NaCl 23915-2
3307 Osmotic Fragility, 0.60 g/dL NaCl 23917-8
3308 Osmotic Fragility, 0.65 g/dL NaCl 23919-4
3309 Osmotic Fragility, 0.75 g/dL NaCl 30543-3
3310 Osmotic Fragility Comment 59466-3

Method Name

Osmotic Lysis

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request Form (T755) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/benign-hematology-test-request-form.pdf)