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Test Code PFGE Bacterial Typing by Pulsed-Field Gel Electrophoresis (PFGE)

Biohazard Infectious

Reporting Name

Bacterial Typing

Useful For

Investigating infection outbreaks by a single bacterial species

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Varies


Advisory Information


This test identifies bacterial strains, except for Staphylococcus aureusStaphylococcus spp., not S. aureus; Listeria spp., Bacillus spp., Enterococcus spp., Streptococcus spp., Lactobacillus spp., Legionella spp., Enterobacteriaceae, non-Enterobacteriaceae, and Clostridium perfringens.

 

For Staphylococcus aureus isolates, order BTWGS / Bacterial Typing by Whole Genome Sequencing.



Shipping Instructions


See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.



Necessary Information


Specimen source and organism identification are required for processing.



Specimen Required


Supplies: Infectious Container, Large (T146)

Container/Tube: Agar slant

Specimen Volume: Isolates on separate agar slants in pure culture

Collection Instructions:

1. Organism must be in pure culture, actively growing. Do not submit mixed cultures.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance.

3. Place all pulsed-field gel electrophoresis (PFGE) specimens (all patients and/or sites to be compared) together in a large bag, and send in the same shipping container. This is necessary for comparison of isolates by this method.

Additional Information:

1. Each slant must be submitted under a separate order.

2. Original isolates should be saved at the client site if additional PFGE testing may be needed (eg, to compare to future outbreak isolates that may occur).


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Reported as isolates from these sources are "indistinguishable" or "different" by pulsed-field gel electrophoresis. Results will be faxed to the client.

Day(s) and Time(s) Performed

Monday through Friday; Continuously

Test Classification

This test uses a standard method. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87152

LOINC Code Information

Test ID Test Order Name Order LOINC Value
PFGE Bacterial Typing In Process

 

Result ID Test Result Name Result LOINC Value
PFGE Bacterial Typing In Process

Method Name

Pulsed-Field Gel Electrophoresis of DNA Fragments