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Test Code OVSRP OXA-48-like (blaOXA-48-like) and VIM (blaVIM) Surveillance, PCR

Useful For

Identifying carriers of Gram-negative bacilli harboring OXA-48-like (oxacillin-hydrolyzing beta-lactamase) or VIM (Verona integron-encoded metallo-beta-lactamase) genes

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

OXA-48 and VIM Surveillance PCR

Specimen Type

Varies


Advisory Information


This assay is for surveillance testing on perirectal/rectal/perianal/anal swabs or stool specimens only. If testing isolates from culture, request OXVRP / OXA-48-like (blaOXA-48-like) and VIM (blaVIM) in Gram-Negative Bacilli, Molecular Detection, PCR.

 

Other mechanisms of carbapenem resistance, including other carbapenemase-types, porin mutations, or hyper-expression of drug efflux pumps may result in carbapenem resistance. These are not detected by this assay. If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is desired, order KNSRP / KPC (blaKPC) and NDM (blaNDM) Surveillance, PCR.



Additional Testing Requirements


If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is also needed; also order KNSRP / KPC (blaKPC) and NDM (blaNDM) Surveillance, PCR.



Necessary Information


Specimen source is required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by oxacillin-hydrolyzing beta-lactamase (OXA-48-like) or Verona integron-encoded metallo-beta-lactamase (VIM) DNA is unlikely.

 

Submit only 1 of the following specimens:

 

Preferred:

Specimen Type: Perianal, perirectal, rectal, anal

Collection Container/Tube: Culture transport swab

 

Acceptable:

Supplies: Cary-Blair or Para-Pak C and S Vial (T058)

Specimen Type: Preserved stool

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of stool

Collection Instructions: Collect fresh stool and submit 1 gram or 5 mL in container with transport medium.


Specimen Stability Information

Specimen Type Temperature Time
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OVSRP OXA-48 and VIM Surveillance PCR 85502-3

 

Result ID Test Result Name Result LOINC Value
OVSRC Specimen Source 31208-2
41746 OXA-48-like PCR 85503-1
41747 VIM PCR 85501-5