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Test Code OVSRP OXA-48-like (blaOXA-48-like) and VIM (blaVIM) Surveillance, PCR, Varies

Useful For

Identifying carriers of Gram-negative bacilli harboring OXA-48-like (oxacillin-hydrolyzing beta-lactamase) or VIM (Verona integron-encoded metallo-beta-lactamase) genes

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

OXA-48 and VIM Surveillance PCR

Specimen Type


Ordering Guidance

This assay is for surveillance testing on perirectal/rectal/perianal/anal swabs or fecal specimens only. If testing isolates from culture, request OXVRP / OXA-48-like (blaOXA-48-like) and VIM (blaVIM) in Gram-Negative Bacilli, Molecular Detection, PCR.


Other mechanisms of carbapenem resistance, including other carbapenemase-types, porin mutations, or hyper-expression of drug efflux pumps may result in carbapenem resistance. These are not detected by this assay. If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is desired, order KNSRP / KPC (blaKPC) and NDM (blaNDM) Surveillance, PCR, Varies.

Additional Testing Requirements

If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is also needed; also order KNSRP / KPC (blaKPC) and NDM (blaNDM) Surveillance, PCR, Varies.

Necessary Information

Specimen source is required.

Specimen Required

The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by oxacillin-hydrolyzing beta-lactamase (OXA-48-like) or Verona integron-encoded metallo-beta-lactamase (VIM) DNA is unlikely.


Submit only 1 of the following specimens:



-Culturette (BBL Culture Swab) (T092)

-C and S Vial (T058)



Specimen Type: Perianal, perirectal, rectal, anal

Collection Container/Tube: Culture transport swab (Dacron or rayon swab with aluminum or plastic shaft with either Stuart or Amies liquid medium)



Supplies: Cary-Blair or Para-Pak C and S Vial (T058)

Specimen Type: Preserved feces

Container/Tube: Commercially available transport system specific for recovery of enteric pathogens from fecal specimens (15 mL of non-nutritive transport medium containing phenol red as a pH indicator, either Cary-Blair or Para-Pak C and S)

Specimen Volume: Representative portion of feces

Collection Instructions: Collect fresh fecal specimen and submit 1 gram or 5 mL in container with transport medium.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Refrigerated (preferred) 7 days
  Frozen  7 days

Reference Values

Not applicable

Day(s) Performed

Monday through Friday

Report Available

1 to 4 days

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OVSRP OXA-48 and VIM Surveillance PCR 85502-3


Result ID Test Result Name Result LOINC Value
OVSRC Specimen Source 31208-2
41746 OXA-48-like PCR 85503-1
41747 VIM PCR 85501-5


If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.