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Test Code OXVRP OXA-48-like (blaOXA-48-like) and VIM (blaVIM) in Gram-Negative Bacilli, Molecular Detection, PCR

Biohazard Infectious

Useful For

Assessing pure isolates of Gram-negative bacilli for mechanism of carbapenem resistance

Method Name

Real-Time Polymerase Chain Reaction (PCR) Using LightCycler and Fluorescent Resonance Energy Transfer (FRET)

Reporting Name

OXA-48 and VIM PCR

Specimen Type

Varies


Advisory Information


This assay should be used for testing of isolates of Gram-negative bacilli. If testing directly from rectal swabs, perirectal swabs, anal swabs, perianal swabs, or stool is desired, request OVSRP / OXA-48-like (blaOXA-48-like) and VIM (blaVIM) Surveillance, PCR.

 

Other mechanisms of carbapenem resistance, including other carbapenemase-types, porin mutations, or hyperexpression of drug efflux pumps may result in carbapenem resistance. These are not detected by this assay. If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is desired, order KPNRP / KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR.



Additional Testing Requirements


1. Organism identification must be provided. If organism identification is unknown, concomitantly order IDENT / Organism Referred for Identification, Aerobic Bacteria.

2. If susceptibility testing is needed; also order ZMMLS / Antimicrobial Susceptibility, Aerobic Bacteria, MIC.

3. If testing for Klebsiella pneumoniae carbapenemase (KPC) or New Delhi metallo-beta-lactamase (NDM) is also needed; also order KPNRP / KPC (blaKPC) and NDM (blaNDM) in Gram-Negative Bacilli, Molecular Detection, PCR.



Shipping Instructions


1. See Infectious Specimen Shipping Guidelines in Special Instructions for shipping information.

2. Place specimen in a large infectious container (T146) and label as an etiologic agent/infectious substance, if appropriate.



Necessary Information


Organism identification and specimen source are required.



Specimen Required


The high sensitivity of amplification by PCR requires the specimen to be processed in an environment in which contamination of the specimen by oxacillin-hydrolyzing beta-lactamase (OXA-48-like) or Verona integron-encoded metallo-beta-lactamase (VIM) DNA is unlikely.

 

Supplies: Infectious Container, Large (T146)

Collection Container/Tube: Slant

Specimen Volume: Isolate

Collection Instructions:

1. Perform isolation of infecting bacteria.

2. Bacterial organism must be submitted in pure culture, actively growing. Do not submit mixed cultures.


Specimen Stability Information

Specimen Type Temperature Time
Varies Ambient (preferred)
  Refrigerated 

Reference Values

Not applicable

Day(s) and Time(s) Performed

Monday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

87798 x 2

LOINC Code Information

Test ID Test Order Name Order LOINC Value
OXVRP OXA-48 and VIM PCR 85502-3

 

Result ID Test Result Name Result LOINC Value
OXORG Organism Identified by Client 43409-2
OXSRC Specimen Source 31208-2
41743 OXA-48-like PCR 85503-1
41744 VIM PCR 85501-5

Forms

If not ordering electronically, complete, print, and send a Microbiology Test Request (T244) with the specimen.