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Test Code LLPT Leukemia/Lymphoma Immunophenotyping, Flow Cytometry, Tissue

Reporting Name

Leukemia Lymphoma Phenotype, Tissue

Useful For

Evaluation of tissues for potential involvement by:

-Chronic lymphoproliferative disorders

-Malignant lymphomas

-Acute lymphoblastic leukemia

-Acute myelogenous leukemia

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
FCINT Flow Cytometry Interp, 2-8 Markers No, (Bill Only) No
FCIMS Flow Cytometry Interp, 9-15 Markers No, (Bill Only) No
FCINS Flow Cytometry Interp,16 or greater No, (Bill Only) No

Testing Algorithm

When this test is ordered, a screening panel and a professional interpretation will always be charged. The screening panel will be charged based on number of makers tested (FIRST for first marker, ADD1 for each additional marker). The interpretation will be set based on markers tested in increments of 9 to 15, or 16 and greater. In addition, reflex testing may occur to fully characterize a disease state or clarify any abnormalities from the screening test. Reflex tests will be performed at an additional charge for each marker tested (FIRST if applicable, ADD1 if applicable).


The tissue panel is initially performed to evaluate for monotypic B-cells by kappa and lambda light chain expression, and increased numbers of blasts and plasma cells by CD45 expression along with side scatter gating. The panel can also evaluate T cells with CD3, CD5, and CD7. Additionally, viability is assessed on all tissue specimens using 7-AAD exclusion.


This panel, together with the provided clinical history and morphologic review, is used to determine what, if any, further testing is needed for disease diagnosis or classification. If additional testing is required, it will be added per algorithm to fully characterize a disease state with a charge per unique antibody tested.


In addition to reflexing flow cytometric panels, fluorescence in situ hybridization (FISH) or molecular testing may be recommended by the Mayo pathologist to facilitate diagnosis. They will contact the referring physician or pathologist to confirm the addition of these tests.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type


Advisory Information


Shipping Instructions

Specimen must arrive within 96 hours of collection.

Necessary Information

1. Date, time of collection, tissue type, and location are required.

2. A pathology/diagnostic report including the client surgical pathology case number, a brief history, reason for testing or clinical suspicion are required before the specimen will be processed.

Specimen Required

Supplies: Hank's Solution (T132)

Container/Tube: Sterile container with 15 mL of tissue culture medium (eg, Hank's balanced salt solution, RPMI, or equivalent)

Specimen Volume: 5 mm(3) or larger biopsy

Collection Instructions:

1. Collect fine-needle aspirate.

2. Send intact specimen (do not mince).

3. Specimen cannot be fixed.

Specimen Minimum Volume

1 mm(3)

Specimen Stability Information

Specimen Type Temperature Time Special Container
Tissue Refrigerated (preferred)

Reference Values

An interpretive report will be provided.

Day(s) and Time(s) Performed

Specimens are processed and reported Monday through Saturday

Test Classification

This test was developed using an analyte specific reagent. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
LLPT Leukemia Lymphoma Phenotype, Tissue In Process


Result ID Test Result Name Result LOINC Value
CK139 LLPT Result No LOINC Needed
19573 Final Diagnosis: 34574-4
19575 Special Studies 30954-2
19571 Microscopic Description 22635-7
19562 Accession Number 57723-9
19563 Referring Pathologist/Physician 46608-6
19564 Ref Path/Phys Address 74221-3
19565 Place of Death: 21987-3
19566 Date and Time of Death: 81956-5
19567 Date of Autopsy: 75711-2
19568 Specimen: 31208-2
19569 Material: 81178-6
19570 Tissue Discription: 22634-0
19572 Clinical History: 22636-5
19574 Final Diagnosis: 34574-4
19576 Revision Description: 81317-0
19577 Signing Pathologist: 19139-5
19578 Special Procedures: 30954-2
19579 SP Signing Pathologist: 19139-5
19580 *Previous Report Follows* 22639-9
19581 Addendum: 35265-8
19582 Addendum Comment: 22638-1
19583 Addendum Pathologist: 19139-5

CPT Code Information

88184-Flow cytometry; first cell surface, cytoplasmic or nuclear marker x 1

88185-Flow cytometry; additional cell surface, cytoplasmic or nuclear marker (each)

88187-Flow Cytometry Interpretation, 2 to 8 Markers (if appropriate)

88188-Flow Cytometry Interpretation, 9 to 15 Markers (if appropriate)

88189-Flow Cytometry Interpretation, 16 or More Markers (if appropriate)

Method Name


Additional Tests

Test ID Reporting Name Available Separately Always Performed
FIRST Flow Cytometry, Cell Surface, First No, (Bill Only) Yes
ADD1 Flow Cytometry, Cell Surface, Addl No, (Bill Only) Yes


1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.