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Test Code MTDNX Methadone Confirmation, Chain of Custody, Random, Urine

Useful For

Monitoring for compliance of methadone treatment for analgesia or drug rehabilitation


Urine measurement of 2-ethylidene-1,5-dimethyl1-3,3-diphenylpyrrolidine is particularly useful for assessing compliance with rehabilitation programs


Chain of custody is required whenever the results of testing could be used in a court of law. Its purpose is to protect the rights of the individual contributing the specimen by demonstrating that it was under the control of personnel involved with testing the specimen at all times; this control implies that the opportunity for specimen tampering would be limited

Additional Tests

Test ID Reporting Name Available Separately Always Performed
COCH Chain of Custody Processing No Yes
ADLTX Adulterants Survey, CoC, U Yes Yes

Testing Algorithm

Adulterants testing will be performed on all chain of custody urine samples as per regulatory requirements.

Reporting Name

Methadone Confirmation, CoC, U

Specimen Type


Specimen Required

Supplies: Chain of Custody Kit (T282)

Container/Tube: Chain-of-Custody Kit containing the specimen containers, seals, and documentation required.

Specimen Volume: 20 mL

Collection Instructions: Collect specimen in the container provided, seal, and submit with the associated documentation to satisfy the legal requirements for chain-of-custody testing.

Additional Information: Submitting <20 mL will compromise our ability to perform all necessary testing.

Specimen Minimum Volume

2.5 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Urine Refrigerated (preferred) 28 days
  Frozen  28 days
  Ambient  14 days

Reference Values



Cutoff concentrations:


<300 ng/mL



<100 ng/mL



<100 ng/mL

Day(s) and Time(s) Performed

Tuesday through Friday

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


G0480 (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
MTDNX Methadone Confirmation, CoC, U In Process


Result ID Test Result Name Result LOINC Value
21105 Methadone Immunoassay Screen 70149-0
36208 EDDP-by GC-MS 58429-2
36209 Methadone-by GC-MS 16199-2
36210 Methadone Interpretation 69050-3
36211 Chain of Custody 77202-0

Method Name

Immunoassay/Gas Chromatography-Mass Spectrometry (GC-MS) Confirmation with Quantitation


1. Chain-of-Custody Request is included in the Chain-of-Custody Kit (T282).

2. If not ordering electronically, complete, print, and send a Therapeutics Test Request (T831) with the specimen.