Specimen Requirements

Patient Preparation
Patient should have nothing to eat or drink for at least 8 to 12 hours before test (except for sips of water). Patient should remain fasting and abstain from smoking, chewing gum, or LifeSavers® during testing period. Patient may not leave during testing period.

After drawing fasting specimen, administer glucose tolerance solution as follows:

Children (by weight)
<20 pounds(9.1 kg): consult  physician

20-24 pounds(9.1-10.9kg): 1.6 ounces

25-29 pounds(11-13.2kg): 2.0 ounces

30-34 pounds(13.3-15.4kg): 2.4 ounces

35-39 pounds(15.5-17.7kg): 2.8 ounces

40-44 pounds(17.8-20kg): 3.2 ounces

45-49 pounds(20.1-22.2kg): 3.6 ounces

50-54 pounds(22.3-24.5kg): 4.0 ounces

55-59 pounds(24.6-26.8kg): 4.4 ounces

60-64 pounds(26.9-29kg): 4.8 ounces

65-69 pounds(29.1-31.3kg): 5.2 ounces

70-74 pounds(31.4-33.6kg): 5.6 ounces

75-79 pounds(33.7-35.8kg): 6.0 ounces

80-84 pounds(35.9-38.1kg): 6.4 ounces

85-89 pounds(38.2-40.4kg): 6.8 ounces

90-94 pounds(40.5-45.6kg): 7.2 ounces

>94 pounds: 7.5 ounces

Adults

Pregnant females: 10.0 ounces (100 g)

Other adults: 7.5 ounces (75 g)

Specimen Collection

Submit only 1 of the following specimens:

Plasma

Container/Tube: Mint-top (lithium heparin) tube

Specimen Volume: 1 mL of plasma for each timed specimen

Collection Instructions: Fasting patient (8-12 hours)

1. After drawing a fasting specimen, give a measured amount of chilled glucose tolerance solution before drawing remaining specimens (patient must drink entire beverage within 5 minutes).

2. Draw specimens at 1-, 2-, and 3-hours for duration of test.

Note: 1. Indicate plasma and draw times.

2. Label specimen appropriately (phlebotomist’s initials, corresponding draw time, and as plasma).

Serum

Container/Tube: Serum gel tube

Specimen Volume: 1 mL of serum for each timed specimen

Collection Instructions: Fasting patient (8-12 hours)

1. After drawing a fasting specimen, give a measured amount of chilled glucose tolerance solution before drawing remaining specimens (patient must drink entire beverage within 5 minutes).

2. Draw specimens at 1-, 2-, and 3-hours for duration of test.

Note: 1. Indicate serum and draw times.

2. Label specimen appropriately (phlebotomist’s initials, corresponding draw time, and as serum).