Test Code WGSEQ Gamma Globin Full Gene Sequencing, Varies
Useful For
An adjunct in the interpretation of hemoglobin electrophoresis results
Evaluation for suspected gamma variants or nondeletional hereditary persistence of fetal hemoglobin (HPFH)
Assess for unstable gamma chain variants (there are occasionally newborns who are jaundiced at birth, often requiring phototherapy, in which all other tests for causes of hemolysis are unrevealing)
Special Instructions
Method Name
Polymerase Chain Reaction (PCR) Amplification/Sanger Sequence Analysis
Reporting Name
Gamma Globin Full Gene SequencingSpecimen Type
VariesShipping Instructions
Specimens must arrive within 30 days (720 hours) of collection.
Necessary Information
A complete patient history is strongly encouraged.
Specimen Required
Submit only 1 of the following specimens:
Specimen Type: Peripheral blood
Container/Tube:
Preferred: Lavender top (EDTA)
Acceptable: Yellow top (ACD)
Specimen Volume: 4 mL
Collection Instructions:
1. Invert several times to mix blood.
2. Send specimen in the original tube.
Specimen Stability: Refrigerate
Specimen Type: Extracted DNA from whole blood
Container/Tube: 1.5-2mL tube with indication of volume and concentration of DNA
Specimen Volume: Entire specimen
Collection Instructions:
1. Label specimen as extracted DNA from blood.
2. Write the DNA concentration on the specimen tube.
Specimen Stability: Frozen (preferred)/Refrigerate/Ambient
Specimen Minimum Volume
Blood: 1 mL; Extracted DNA: 50 mcL at 50 ng/mcL concentration
Specimen Stability Information
Specimen Type | Temperature | Time |
---|---|---|
Varies | Varies | 30 days |
Reference Values
An interpretive report will be provided.
Day(s) and Time(s) Performed
Monday through Friday
Performing Laboratory

Test Classification
This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.CPT Code Information
81479-Unlisted molecular
LOINC Code Information
Test ID | Test Order Name | Order LOINC Value |
---|---|---|
WGSEQ | Gamma Globin Full Gene Sequencing | In Process |
Result ID | Test Result Name | Result LOINC Value |
---|---|---|
46952 | Gamma Globin Gene Sequencing Result | In Process |
46953 | Gamma Globin Interpretation | In Process |
Forms
1. Thalassemia/Hemoglobinopathy Patient Information (T358) in Special Instructions
2. New York Clients-Informed consent is required. Please document on the request form or electronic order that a copy is on file. An Informed Consent for Genetic Testing (T576) in Special Instructions.