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HelpTest Code Z0115 von Willebrand Factor Activity, Plasma
Additional Codes
Mayo: VWACT
Ordering Guidance
This activity assay is most effective when it is combined with measurement of von Willebrand factor antigen and factor VIII coagulant activity, preferably as a panel of tests with reflexive testing and interpretive reporting. See AVWPR / von Willebrand Disease Profile, Plasma.
Additional Testing Requirements
Tests for F8A / Coagulation Factor VIII Activity Assay, Plasma and VWAG / von Willebrand Factor Antigen, Plasma are recommended in conjunction with this test (von Willebrand activity).
Specimen Required
Specimen Type: Platelet-poor plasma
Collection Container/Tube: Light-blue top (3.2% sodium citrate)
Submission Container/Tube: Plastic vial (polypropylene preferred)
Specimen Volume: 2 mL in 2 vials each containing 1 mL
Collection Instructions:
1. Specimen must be collected prior to factor replacement therapy.
2. For complete instructions, see Coagulation Guidelines for Specimen Handling and Processing.
3. Centrifuge, transfer all plasma into a polypropylene vial, and centrifuge plasma again.
4. Aliquot plasma (1-2 mL per aliquot) into 2 separate polypropylene vials leaving 0.25 mL in the bottom of centrifuged vial.
5. Freeze plasma immediately (no longer than 4 hours after collection) at -20° C or, ideally, ≤-40° C.
6. Send specimens in the same shipping container.
Additional Information:
1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.
2. Each coagulation assay requested should have its own vial.
Useful For
Diagnosis of von Willebrand disease (VWD) and differentiation of VWD subtypes or differentiation of VWD from hemophilia A
Monitoring therapeutic efficacy of treatment with DDAVP (desmopressin) or VWF concentrates in patients with VWD
Reflex Tests
| Test ID | Reporting Name | Available Separately | Always Performed |
|---|---|---|---|
| RIST | Ristocetin Cofactor, P | No | No |
Testing Algorithm
If von Willebrand factor activity is less than 55%, then the von Willebrand factor ristocetin cofactor activity assay will be performed at an additional charge.
Special Instructions
Method Name
Latex Immunoassay
Reporting Name
von Willebrand Factor Activity, PSpecimen Type
Plasma Na CitSpecimen Minimum Volume
0.5 mL
Specimen Stability Information
| Specimen Type | Temperature | Time | Special Container |
|---|---|---|---|
| Plasma Na Cit | Frozen | 14 days |
Reference Values
55-200%
Normal, full-term newborn infants may have mildly increased levels which reach adult levels by 90 days postnatal. Healthy, premature infants (30-36 weeks gestation) may have increased levels that reach adult levels by 180 days.
Note: Individuals of blood group "O" may have lower plasma von Willebrand factor (VWF) activity than those of other ABO blood groups, such that apparently normal individuals of blood group "O" may have plasma VWF activity as low as 40% to 50%, whereas the lower limit of the reference range for individuals of other blood groups may be 60% to 70%.
Day(s) Performed
Monday through Saturday
Report Available
1 to 3 daysPerforming Laboratory
Mayo Clinic Laboratories in Rochester
Test Classification
This test has been modified from the manufacturer's instructions. Its performance characteristics were determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.CPT Code Information
85397
LOINC Code Information
| Test ID | Test Order Name | Order LOINC Value |
|---|---|---|
| VWACT | von Willebrand Factor Activity, P | 68324-3 |
| Result ID | Test Result Name | Result LOINC Value |
|---|---|---|
| VWACT | von Willebrand Factor Activity, P | 68324-3 |
Forms
If not ordering electronically, complete, print, and send a Coagulation Test Request (T753) with the specimen.